Moderne Infrastruktur für genetische, molekulare und bildgebende Analysen steht für die Wissenschaftler*innen des DZMB aber auch für externe Nutzer*innen und Kooperationspartner*innen zur Verfügung.
Unser Schwerpunkt liegt auf der Weiterentwicklung der molekularen Biodiversitätsforschung durch die Integration genomischer Ansätze, naturkundlicher Sammlungen und ökologischer Anwendungen. Ziel ist es, klassische Taxonomie mit modernen molekularen Methoden zu verbinden, um Biodiversitätsmuster, evolutionäre Prozesse und die Reaktionen von Ökosystemen auf Umweltveränderungen besser zu verstehen.
Marine Ökosysteme, insbesondere benthische Fauna und Zooplankton Gemeinschaften, gehören aufgrund ihrer enormen Diversität, der geringen Körpergröße vieler Organismen und des Mangels an taxonomischer Expertise zu den am wenigsten verstandenen Komponenten der Biodiversität. Wir begegnen diesen Herausforderungen durch die Entwicklung skalierbarer molekularer Ansätze, die eine schnelle und hochauflösende Charakterisierung biologischer Gemeinschaften über verschiedene Lebensräume hinweg ermöglichen – von Küstensystemen bis in die Tiefsee.
In diesem Kontext baut unsere Arbeit auf zwei komplementären Säulen auf. Erstens entwickeln und wenden wir molekulare Monitoring-Ansätze auf Gemeinschaftsebene an, insbesondere DNA-Metabarcoding und Umwelt-DNA (eDNA), um Veränderungen der Biodiversität unter natürlichen und anthropogenen Einflüssen zu erfassen. Zweitens generieren wir sammlungsbasierte molekulare Ressourcen, darunter kuratierte DNA-Barcoding-Referenzbibliotheken sowie genomische Datensätze aus frisch gewonnenem und historischem Material. Durch die Integration von typenbasiertem Barcoding in molekulare Workflows decken wir kryptische Diversität auf und lösen taxonomische Unklarheiten.
Ein zentraler Bestandteil unserer Forschung ist die kontinuierliche Weiterentwicklung von Labor- und bioinformatischen Workflows. Wir optimieren Protokolle für Sequenzierungen und die Erstellung von NGS-Bibliotheken und entwickeln reproduzierbare bioinformatische Pipelines zur Verarbeitung von Hochdurchsatz-Sequenzdaten, einschließlich Trimming, Filterung und taxonomischer Zuordnung. Unsere Arbeiten konzentrieren sich auf weit verbreitete genetische Marker wie das mitochondriale COI-Gen sowie die hypervariablen Regionen der 18S rRNA, und werden durch phylogenomische Ansätze erweitert, bei denen Phylotranskriptomik eingesetzt wird, um tiefgreifende evolutionäre Beziehungen aufzuklären und taxonomische Systeme über verschiedene marine Taxa hinweg zu verfeinern.
Über die reine Biodiversitätserfassung hinaus untersuchen wir genetische Konnektivität und evolutionäre Dynamiken mithilfe genomischer Methoden wie 2bRAD-Sequenzierung und Mitogenomik. Diese Ansätze ermöglichen es, genetische Strukturmuster mit ökologischen Prozessen zu verknüpfen und eine wissenschaftliche Grundlage für Naturschutz- und Managementstrategien zu schaffen.
Eine besondere Stärke unserer Einheit liegt in ihrem integrativen Ansatz. Wir verbinden molekulare Daten mit morphologischer Expertise durch enge Zusammenarbeit mit Taxonominnen und Taxonomen und gewährleisten so hochwertige Referenzsysteme für die Artidentifikation, während wir gleichzeitig aktiv zur methodischen Weiterentwicklung beitragen.
Durch diesen Ansatz entwickelt unsere Arbeitsgruppe robuste, skalierbare und zukunftsorientierte Werkzeuge für die Biodiversitätsforschung. Durch die Einbettung molekularer Methoden in sammlungsbasierte Forschung und kooperative Netzwerke tragen wir sowohl zur Grundlagenforschung als auch zum angewandten Monitoring bei und leisten damit einen wichtigen Beitrag zum Verständnis und Schutz mariner Ökosysteme.
2026
Kunze M., Khodami S., Ostmann A., Packmor J., George K.H. (2026) Redescription of Enhydrosoma sarsi (Scott, 1905) (Copepoda, Harpacticoida, Cletodidae T. Scott) from the western Baltic Sea (Germany) and remarks on the systematics of Enhydrosoma Boeck, 1873. Zootaxa, 5768 (3), 335–369. https://doi.org/10.11646/zootaxa.5768.3.2
Damm L., Khodami S., Rubel V., Molari M., Vink A., Stoeck T., Martinez Arbizu P. (2026) Community DNA outperforms eDNA metabarcoding for biodiversity assessments in the Clarion–Clipperton Fracture Zone. Metabarcoding and Metagenomics 10: e173793. https://doi.org/10.3897/mbmg.10.173793
Gwinner M., Haslob H., Neumann H., Schupp P.J., Khodami S., Bonthond G. (2026) Flatfish intestinal microbiota depend on various host traits, and vary with sediment type and bottom trawling effort. Scientific Reports 16, 632. https://doi.org/10.1038/s41598-025-34195-w
2025
Stratmann T., Simon-Lledó E., van der Meer M., Christodoulou M., Rossel S., Colaco A. (2025) Trophic ecology of Ophiuroidea and Asteroidea in the Clarion-Clipperton-Fracture Zone (Central Pacific), Deep Sea Research Part I: Oceanographic Research Papers 225, https://doi.org/10.1016/j.dsr.2025.104598
Blanco-Bercial L., Questel M.J., Batta-Lona P., Escribano R., Falkenhaug T., Hirai J., Huggett J., Martínez Arbizu P., Peijnenburg K., Suter L., Weydmann-Zwolicka A., Dubbeldam S., Duijm E., Ershova E., González C.E., Govender A., Groeneveld J., Khodami S., Kuczyński R., MacDonald A., Mioduchowska M., Polanowski A.M., Rodríguez-Pérez R., O’Brien T., Bucklin A. (2025) MetaZooGene Intercalibration Experiment (MZG-ICE): Metabarcoding marine zooplankton diversity of the global Ocean. Molecular Ecology Resources 26, 1: e70090. https://doi.org/10.1111/1755-0998.70090
Khodami S., Ostmann A., Packmor J., George K.H., Martínez Arbizu P. (2025) Baseline studies on meiofauna in the Baltic Sea before bottom-trawl fisheries exclusion II. A comparative study using multi-gene metabarcoding and morphology. Metabarcoding and Metagenomics 9: e169630. https://doi.org/10.3897/mbmg.9.169630
Bonk F., Khodami S., Brandt A., Martínez Arbizu P., Hadal copepods in and around: A metabarcoding Survey of meiofauna in the Aleutian trench and adjacent regions, Progress in Oceanography 239: 103596. https://doi.org/10.1016/j.pocean.2025.103596
Christodoulou M., Derycke S., Beentjes K.K., Hillewaert H., Laakmann S., Lundin K., Kamyab E., Khodami S., Maes S., Reiss H., Uhlir C., Van den Bulcke L., Van der Hoorn B., De Backer A., Martinez Arbizu P. (2025) A taxonomically reliable DNA barcode reference library for North Sea macrobenthos. Scientific Data 12, 1198. https://doi.org/10.1038/s41597-025-05500-z
Bernot J.P., Khodami S., Boyen J., de Troch M., Boxshall G.A., Martínez Arbizu P. (2025) Copepod phylogenomics supports Canuelloida as a valid order separate from Harpacticoida, Molecular Phylogenetics and Evolution 206, 108311. https://doi.org/10.1016/j.ympev.2025.108311
Neuhaus, J., deWilt M. E., Rossel S., et al. (2025) “High Connectivity at Abyssal Depths: Genomic and Proteomic Insights Into Population Structure of the Pan-Atlantic Deep-Sea Bivalve Ledella ultima (E. A. Smith, 1885).” Ecology and Evolution15, 8: e71903. https://doi.org/10.1002/ece3.71903
Wieschermann L., Kihara T.C. (2025) A new species of the genus Aphotopontius Humes, 1987 (Siphonostomatoida, Dirivultidae) from a vent field at the Southeast Indian Ridge, Plankton and Benthos Research 20, s120, https://doi.org/10.3800/pbr.20.s102
Kuru S., Martínez Arbizu P., Rossel S. (2025) Revealing high genetic divergence masked by low morphological variability in harpacticoid genus Leptastacus Scott T., 1906 (Copepoda, Harpacticoida, Leptastacidae) including the description of five new species. Marine Biodiversity 55:113. https://doi.org/10.1007/s12526-025-01583-4
Kniesz K., Hoffman L., Martínez Arbizu P., Kihara T.C. (2025) High genomic connectivity within Anatoma at hydrothermal vents along the Central and Southeast Indian Ridge. Scientific Report 15, 1971. https://doi.org/10.1038/s41598-025-85507-z
2024
Reñé A., Timoneda N., Khodami S., López-García P., Martinez Arbizu P., Hoppenrath M. (2024) Morpho-molecular characterization of sand-dwelling dinoflagellate communities from the German Wadden Sea and insights into their spatiotemporal distribution. European Journal of Phycology, 59 (2), 196–217. https://doi.org/10.1080/09670262.2023.2279547
Neufeld M., Meyn K., Kihara T.C., Martinez Arbizu P., Kuhn T. (2024) First record of the giant anemone, Relicanthus daphneae, at active hydrothermal vent fields in the Indian Ocean. Journal of the Marine Biological Association of the United Kingdom 104, e122, 1–6. https://doi.org/10.1017/ S0025315424001127
Kapshyna I., Veit-Köhler G., Hoffman L., Khodami S. (2024) Impact of a coastal protection measure on sandy-beach meiofauna at Ahrenshoop (Baltic Sea, Germany): results from metabarcoding and morphological approaches are similar. Metabarcoding and Metagenomics 8: e127688. https://https://doi.org/10.3897/mbmg.8.127688
Sosa SOSA, Brandt A, Chen C, Engel L, Esquete P, Horton T, Jażdżewska AM, Johannsen N, Kaiser S, Kihara TC, Knauber H, Kniesz K, Landschoff J, Lörz AN, Machado FM, Martínez-Muñoz CA, Riehl T, Serpell-Stevens A, Sigwart JD, Tandberg AHS, Tato R, Tsuda M, Vončina K, Watanabe HK, Wenz C, Williams JD. (2024) Ocean Species Discoveries 1-12 – A primer for accelerating marine invertebrate taxonomy. Biodiversity Data Journal 12: e128431. https://doi.org/10.3897/BDJ.12.e128431
Stratmann T., Busch K., de Kluijver A., Kelly M., Mills S., Rossel S., Schupp P.J., (2024) Nutrient fluxes, oxygen consumption and fatty acid composition from deep-water demo- and hexactinellid sponges from New Zealand, Deep Sea Research Part I: Oceanographic Research Papers b 214, 104416, https://doi.org/10.1016/j.dsr.2024.104416
2023
Bonthond G., Beermann J., Gutow L., Neumann A., Barboza F., Desiderato A., Fofonova V., Helber S., Khodami S., Kraan C., Neumann H., Rohde S., Schupp P. (2023) Benthic microbial biogeographic trends in the North Sea are shaped by an interplay of environmental drivers and bottom trawling effort. ISME Communications 3, 132. https://doi.org/10.1038/s43705-023-00336-3
Eichsteller A., Martynov A., O’Hara T.D., Christodoulou M., Korshunova T., Bribiesca-Contreras G., Martinez Arbizu P. (2023) Ophiotholia (Echinodermata: Ophiuroidea): A little-known deep-sea genus present in polymetallic nodule fields with the description of a new species. Frontiers in Marine Science. 10:1056282. https://doi.org/10.3389/fmars.2023.1056282
Kaiser S., Christodoulou M., Janssen A., Kihara T.C., Mohrbeck I., Pasotti F., Schnurr S., Vink A., Martinez Arbizu P. (2023) Diversity, distribution and composition of abyssal benthic Isopoda in a region proposed for deep-seafloor mining of polymetallic nodules: a synthesis. Marine. Biodiversity. 53, 30. https://doi.org/10.1007/s12526-023-01335-2
Hablützel P.I., Arbizu Martínez P., Bilsen A., Christodoulou M., Delacauw S., Deneudt K., Khodami S., et al. (2023) DNA-based monitoring of non-Indigenous Species (NIS) Project: GEANS-Genetic tools for Ecosystem health Assessment in the North Sea region. [Technical report] https://doi.org/10.13140/RG.2.2.23641.53606
Derycke S., Beentjes K., Christodoulou M, Hansen J, Khodami S., Kröncke I., Martinez Arbizu P., et al. (2023) DNA-Based monitoring of soft sediments. Project: GEANS-Genetic tools for Ecosystem health Assessment in the North Sea region. [Technical report] https://doi.org/10.13140/RG.2.2.30352.42241
2022
Rossel S., Kaiser P., Bode-dalby M., Renz J., Laakmann S., Auel H., Hagen W., Arbizu P.M., Peters J. (2022) Proteomic fingerprinting enables quantitative biodiversity assessments of species and ontogenetic stages in Calanus congeners (Copepoda, Crustacea) from the Arctic Ocean. Molecular Ecology Resources. 23, 382–395. https://doi.org/10.1111/1755-0998.13714
Rossel S., Uhlenkott K., Peters J., Vink A., Martínez Arbizu P. (2022) Evaluating species richness using proteomic fingerprinting and DNA barcoding—a case study on meiobenthic copepods from the Clarion Clipperton Fracture Zone, Marine Biodiversity 52 (6), https://doi.org/10.1007/s12526-022-01307-y
Ralf T., Christodoulou M., Pogonoski J., Weddehage T., Vink A., Martinez Arbizu P. (2022) An application of morphological analysis and DNA barcoding to identify Ipnops from the Clarion-Clipperton Zone (CCZ) as I. meadi Nielsen, 1966 with notes on other species of the genus (Aulopiformes: Ipnopidae), Marine Biodiversity 52, https://doi.org/10.1007/s12526-022-01320-1
Hoffmann, L., Kniesz K., Martinez Arbizu, P., Kihara T.C. (2022) Abyssal vent field habitats along plate margins in the Central Indian Ocean yield new species in the genus Anatoma (Vetigastropoda: Anatomidae) European Journal of Taxonomy 826:135-162. https://doi.org/10.5852/ejt.2022.826.1841
Rossel S., Kaiser P., Bode-dalby M., Renz J., Laakmann S., Auel H., Hagen W., Martínez Arbizu P., Peters J. (2022) Proteomic fingerprinting enables quantitative biodiversity assessments of species and ontogenetic stages in Calanus congeners (Copepoda, Crustacea) from the Arctic Ocean. Molecular Ecology Resources 23, 2, https://doi.org/10.1111/1755-0998.13714
Christodoulou M., Grave S., Vink A., Martinez Arbizu P. (2022) Taxonomic assessment of deep-sea decapod crustaceans collected from polymetallic nodule fields of the East Pacific Ocean using an integrative approach, Marine Biodiversity 52, https://doi.org/10.1007/s12526-022-01284-2
Kniesz K., Jażdżewska A.M., Martínez Arbizu P., Kihara T.C. (2022) DNA Barcoding of Scavenging Amphipod Communities at Active and Inactive Hydrothermal Vents in the Indian Ocean. Frontiers in Marine Science. 8:752360. https://doi.org/10.3389/fmars.2021.752360
Korfhage S., Rossel S., Brix S., Mcfadden C.S., Martínez Abizu P. (2022) Species delimitation of Hexacorallia and Octocorallia around Iceland using nuclear and mitochondrial DNA and proteome fingerprinting, Frontiers in Marine Science, Deep-Sea Environments and Ecology 9. https://doi.org/10.3389/fmars.2022.838201
Sánchez N., González Casarrubios A., Cepeda D., Khodami S., Pardos F., Vink A., Martinez Arbizu P. (2022) Diversity and distribution of Kinorhyncha in abyssal polymetallic nodule areas of the Clarion-Clipperton Fracture Zone and the Peru Basin, East Pacific Ocean, with the description of three new species and notes on their intraspecific variation. Marine Biodiversity 52. https://link.springer.com/article/10.1007/s12526-022-01279-z
Paulus E., Brix E., Siebert A., Martinez Arbizu P., Rossel S., Peters J., Svavarsson J., Schwentner M. (2022) A complex pattern of recent speciation and hybridization in a deep-sea benthic isopod species-complex around Iceland revealed by DNA barcoding, ddRAD and proteomics. Molecular Ecology 31:313-330, https://doi.org/10.1111/mec.16234
2021
Jazdzewska A., Horton T., Hendrycks E., Mamos T., Driskell A., Brix S., Martinez Arbizu P. (2021) Pandora’s box in the deep sea –intraspecific diversity patterns and distribution of two congeneric scavenging amphipods. Frontiers in Marine Science, Sec. Deep-Sea Environments and Ecology. https://doi.org/10.3389/fmars.2021.750180
Rent J., Marksaseva E.L., Laakmann S., Rossel S., Martinez Arbizu P., Peters J. (2021) Molecular Ecology Resources; Proteomic fingerprinting facilitates biodiversity assessments in understudied ecosystems: a case study on integrated taxonomy of deep-sea copepods. Molecular Ecology Resources 21, 6, https://doi.org/10.1111/1755-0998.13405
Demidow O., Kihara T.C., Martinez Arbizu P., Clark P.F. (2021) The megalopal stage of the hydrothermal vent crab Austinograea rodriguezensis Tsuchida & Hashimoto, 2002 (Decapoda: Bythograeidae): a morphological description based on CLSM images. Zootaxa 5040(3):365-387, https://doi.org/10.11646/ZOOTAXA.5040.3.3
Thiel R., Knebelsberger T., KiharaI T.C., Gerdes K. (2021) Description and DNA barcoding of a new eelpout Pachycara angeloi sp. nov. (Perciformes: Zoarcidae) from deep-sea hydrothermal vent fields in the Indian Ocean. Zootaxa 4980(1):99-112, https://doi.org/10.11646/zootaxa.4980.1.6
Kaiser S., Kihara T.C., Brix S., Mohrbeck I., Janssen A., Jennings R. (2021) Species boundaries and phylogeographic patterns in new species of Nannoniscus (Janiroidea: Nannoniscidae) from the equatorial Pacific nodule province inferred from mtDNA and morphology. Zoological Journal of the Linnean Society 193(3), https://doi.org/10.1093/zoolinnean/zlaa174
Degenhardt J., Khodami S., Milke F., Waska H., Engelen B., Martinez Arbizu P. (2021) The Three Domains of Life Within the Discharge Area of a Shallow Subterranean Estuary at a High Energy Beach. Frontiers in Environmental Science. 9. 642098. https://doi.org/10.3389/fenvs.2021.642098
Mohrbeck I., Horton T., Jażdżewska A., Martínez Arbizu P. (2021) DNA-barcoding and Cryptic Diversity of Deep-Sea Scavenging Amphipods in the Clarion-Clipperton Zone (Eastern Equatorial Pacific). Marine Biodiversity, 51, 26. https://doi.org/10.1007/s12526-021-01170-3
Jażdżewska A.M., Brandt A., Martinez Arbizu P., Vink A. (2021) Exploring the diversity of the deep sea – four new species of the amphipod genus Oedicerina described using morphological and molecular methods. Zoological Journal of the Linnean Society 194(1), https://doi.org/10.1093/zoolinnean/zlab032
2020 72. Rossel S., Barco A., Kloppmann M., Martínez Arbizu P., Huwer B., Knebelsberger T. (2020) Rapid species level identification of fish eggs by proteome fingerprinting using MALDi-TOF MS“.Journal of Proteomics https://doi.org/10.1016/j.jprot.2020.103993
Brix S., Osborn K.J., Kaiser S., Truskey S.B., Schnurr S.M., Brenke N., Malyutina M., Martínez Arbizu P. (2020) Adult life strategy affects distribution patterns in abyssal isopods – implications for conservation in Pacific nodule areas. Biogeosciences 17(23), https://doi.org/10.5194/bg-17-6163-2020
Rahlff J., Khodami S., Voskuhl L., Humphreys M., Stolle C., Martínez-Arbizu P., Wurl, O., Ribas-Ribas M. (2021) Short-term responses to ocean acidification: effects on relative abundance of eukaryotic plankton from the tropical Timor Sea. Marine Ecology Progress Series. 658. 59-74. https://doi.org/10.3354/meps13561
Steinert G., Busch K., Bayer K., Kodami S., Martinez Arbizu P., Kelly M., Mills S., Erpenbeck D., Dohrmann M., Wörheide G., Hentschel U., Schupp P.J. (2020) Compositional and Quantitative Insights Into Bacterial and Archaeal Communities of South Pacific Deep-Sea Sponges (Demospongiae and Hexactinellida). Frontiers in microbiology. https://doi.org/10.3389/fmicb.2020.00716
Christodoulou M., O’hara T., Hugall A., Khodami S., Rodrigues C.F., Hilario A., Vink A., Martinez Arbizu P. (2020) Unexpected high abyssal ophiuroid diversity in polymetallic nodule fields of the northeast Pacific Ocean and implications for conservation. Biogeosciences, 17, 1-32. https://doi.org/10.5194/bg-17-1-2020
Mercado-Salas N., Khodami S., Martínez Arbizu P. (2021) Copepods and ostracods associated with bromeliads in the Yucatán Peninsula, Mexico. PLOS ONE. 16. https://doi.org/10.1371/journal.pone.0248863
Khodami S., Mercado-Salas N., Martínez Arbizu P. (2020) Genus level molecular phylogeny of Aegisthidae Giesbrecht, 1893 (Copepoda: Harpacticoida) reveals morphological adaptations to deep-sea and pelagic habitats. BMC Evolutionary Biology. 20. 36. https://doi.org/10.1186/s12862-020-1594-x
2019
Rossel S., Khodami S., Martínez Arbizu P. (2019) Comparison of Rapid Biodiversity Assessment of Meiobenthos Using MALDI-TOF MS and Metabarcoding. Frontiers in Marine Science 6:659. https://doi.org/10.3389/fmars.2019.00659
Christodoulou M., O’hara T., Hugall A.F., Martinez Arbizu P. (2019) Dark Ophiuroid Biodiversity in a Prospective Abyssal Mine Field. Current Biology 29, 1-4. https://doi.org/10.1016/j.cub.2019.09.012
Rossel S., Martínez Arbizu P. (2019) Revealing higher than expected diversity of Harpacticoida (Crustacea: Copepoda) in the North Sea using MALDI-TOF MS and molecular barcoding. Scientific Reports. https://doi.org/10.1038/s41598-019-45718-7
Khodami S., Mercado-salas N.F., Tang D., Martinez Arbizu P. (2019) Molecular evidence for the retention of the Thaumatopsyllidae in the order Cyclopoida (Copepoda) and establishment of four suborders and two families within the Cyclopoida. Molecular Phylogenetics and Evolution, 138:43-52. https://doi.org/10.1016/j.ympev.2019.05.01
Holst S., Heins A., Laakmann S. (2019) Morphological and molecular diagnostic species characters of Staurozoa (Cnidaria) collected on the coast of Helgoland (German Bight, North Sea). 10013/epic.4f95b184-bb12-44fe-943d-b0f989f1bf93
Laakmann S., Markhaseva E.L., Renz J. (2019) Do molecular phylogenies unravel the relationships among the evolutionary young “Brafordian” families (Copepoda; Calanoida)? Molecular Phylogenetics and Evolution, 130: 330-345. https://doi.org/10.1016/j.ympev.2018.10.028
Holst, S., Laakmann, S. (2019) First record of the stalked jellyfish Haliclystus tenuis Kishinouye, 1910 (Cnidaria: Staurozoa) in Atlantic waters. Marine Biodiversity 49, 1061–1066. https://doi.org/10.1007/s12526-018-0888-3
2018
Mercado-Salas N.F., Mercado-Salas N.F., Khodami S., Kihara T.C., Elías-Gutiérrez M., Arbizu P.M. (2018) Genetic structure and distributional patterns of the genus Mastigodiaptomus (Copepoda) in Mexico, with the description of a new species from the Yucatan Peninsula. Arthropod Systematics & Phylogeny 76(3): 487-507. https://doi.org/10.3897/asp.76.e31965
Christodoulou M., Cristina Kihara T. (2018) Lectotype designation and distribution updates on the freshwater shrimp species Atyaephyra stankoi Karaman 1972. Zootaxa 4531 (1): 123–133. https://doi.org/10.11646/zootaxa.4531.1.7
Renz J., Markhaseva E.L., Laakmann S. (2018) The phylogeny of Ryocalanoidea (Copepoda, Calanoida) based on morphology and a multi-gene analysis with a description of new ryocalanoidean species. Zoological Journal of the Linnean Society, 1-33 . https://doi.org/10.1093/zoolinnean/zly069
Rossel S., Martinez Arbizu P. (2018) Effects of Sample Fixation on Specimen Identification in Biodiversity Assemblies Based on Proteomic Data (MALDI-TOF). Frontiers in Marine Science 5:149. https://doi.org/10.3389/fmars.2018.00149
Mercado-Salas N., Martínez Arbizu P., Khodami S. (2018) Convergent evolution of mouthparts morphology between Siphonostomatoida and a new genus of deep-sea Aegisthidae Giesbrecht, 1893 (Copepoda: Harpacticoida). Arthropod Systematics and Phylogeny 76(3). 487-507. https://doi.org/10.1007/s12526-018-0932-3
Günther B., Knebelsberger T., Neumann H., Laakmann S., Martinez Arbizu P. (2018) Metabarcoding of marine environmental DNA based on mitochondrial and nuclear genes. Scientific Reports 8, 14822. https://doi.org/10.1038/s41598-018-32917-x
Mertens K.N., Carbonell-Moore M.C., Pospelova V., Head M.J., Highfield A., Schroeder D., Gu H., Andree K.B., Fernandez M., Yamaguchi A., Takano Y., Matsuoka K., Nézan E., Bilien G., Okolodkov Y., Koike K., Hoppenrath M., Pfaff M., Pitcher G., Al-Muftah A., Rochon A., Lim P.T., Leaw C.P., Lim Z.F., Ellegaard M., 2018. Pentaplacodinium saltonense gen. et sp. nov. (Dinophyceae) and its relationship to the cyst-defined genus Operculodinium and yessotoxin-producing Protoceratium reticulatum. Harmful Algae, 71: 57-77. https://doi.org/10.1016/j.hal.2017.12.003
2017
Günther B., Raupach M.J., Knebelsberger T. (2016) Full-length and mini-length DNA barcoding for the identification of seafood commercially traded in Germany. Food Control 73, 922-929. https://doi.org/10.1016/j.foodcont.2016.10.016
Tillmann U., Hoppenrath M., Gottschling M., Kusber W.H. Elbrächter M. (2017) Plate pattern clarification of the marine dinophyte Heterocapsa triquetra sensu Stein (Dinophyceae) collected at the Kiel Fjord (Germany). Journal of Phycology 53: 1305-1324. https://doi.org/10.1111/jpy.12584
Laakmann S., Boos K., Knebelsberger T., Raupach M.J., Neumann H. (2017) Species identification of echinoderms from the North Sea by combining morphology and molecular data. Helgoland Marine Research, 70: 18. https://doi.org/10.1186/s10152-016-0468-5
Kieneke A., Nikoukar H. (2017) Integrative morphological and molecular investigation of Turbanella hyalina Schultze, 1853 (Gastrotricha: Macrodasyida), including a redescription of the species. Zoologischer Anzeiger, 267: 168–186. https://doi.org/10.1016/j.jcz.2017.03.005
Hoppenrath M., Yubuki N., Stern R., Leander B.S. (2017) Ultrastructure and molecular phylogenetic position of a new marine sand-dwelling dinoflagellate from British Columbia, Canada: Pseudadenoides polypyrenoides sp. nov. (Dinophyceae). European Journal of Phycology, 52(2), 208–224. https://doi.org/10.1080/09670262.2016.1274788
Hofmann T., Knebelsberger T., Kloppmann M., Ulleweit J., Raupach M.J. (2017) Egg identification of three economical important fish species using DNA barcoding in comparison to a morphological determination. Journal of Applied Ichthyology, 33: 925–932. https://doi.org/10.1111/jai.13389
Bode M., Laakmann S., Kaiser P., Hagen W., Auel H., Cornils A. (2017) Unravelling diversity of deep-sea copepods using integrated morphological and molecular techniques. Journal of Plankton Research, 39: 600–617. https://doi.org/10.1093/plankt/fbx031
2016
Gollner S., Stuckas H., Kihara T.C., Laurent S., Khodami S., Martínez Arbizu P. (2016) Mitochondrial DNA analyses indicate high diversity, expansive population growth and high genetic connectivity of vent copepods (Dirivultidae) across different oceans. PLOS ONE. 11 (10). https://doi.org/10.1371/journal.pone.0163776
Thiel R., Knebelsberger T. (2016) How reliably can northeast Atlantic sand lances of the genera Ammodytes and Hyperoplus be distinguished? A comparative application of morphological and molecular methods. ZooKeys, 617, 139-164, https://doi.org/10.3897/zookeys.617.8866
Schade F.M., Raupach M.J., Wegner K.M. (2016) Seasonal variation in parasite infection patterns of marine fish species from the Northern Wadden Sea in relation of interannual temperature fluctuations. Journal of the Sea 113: 73-84. https://doi.org/10.1016/j.seares.2015.09.002
Raupach M.J., Amann R., Wheeler Q. et al. (2016) The application of “-omics” technologies for the classification and identification of animals. Organisms Diversity & Evolution, 16: 1–12. https://doi.org/10.1007/s13127-015-0234-6
Oliveira L.M., Knebelsberger T., Landi M., Soares P., Raupach M.J., Costa F.O. (2016) Assembling and auditing a comprehensive DNA barcode reference library for European marine fishes. Journal of Fish Biology, 89: 2741–2754. https://doi.org/10.1111/jfb.13169
Miljutin D.M., Miljutina M.A. (2016) Intraspecific variability of morphological characters in the species-rich deep-sea genus Acantholaimus Allgén, 1933 (Nematoda: Chromadoridae). Nematology, 18(4), 455-473. https://doi.org/10.1163/15685411-00002970
Meissner K., Bick A., Götting M. (2016) Arctic Pholoe (Polychaeta, Pholoidae): when integrative taxonomy helps to sort out barcodes. Zoological Journal of the Linnean Society. https://doi.org/10.1111/zoj.12468
Laakmann S., Boos K., Knebelsberger T., Raupach M.J., Neumann H. (2016) Species identification of echinoderms from the North Sea by combining morphology and molecular data. Helgoland Marine Research 70:18. https://doi.org/10.1186/s10152-016-0468-5
Barco A., Raupach M.J., Laakmann S., Neumann H., Knebelsberger T. (2016) Identification of North Sea molluscs with DNA barcoding. Molecular Ecology Resources, 16: 288–297. https://doi.org/10.1111/1755-0998.12440
2015
Raupach M.J., Barco A., Steinke D., Beermann J., Laakmann S., Mohrbeck I., Neumann H., Kihara T.C., Pointner K., Radulovici A., Segelken-voigt A., Wesse C., Knebelsberger T. (2015) The application of DNA barcodes for the identification of marine crustaceans from the North Sea and adjacent regions. Public Library of Science ONE 10 (9): e0139421. https://doi.org/10.1371/journal.pone.0139421
Raupach M.J., Radulovici A.E. (2015) Looking back on a decade of barcoding crustaceans. Zookeys. 23 (539), 53-81. https://doi.org/10.3897/zookeys.539.6530.
Mohrbeck I., Raupach M.J., Martínez Arbizu P., Knebelsberger T., Laakmann S. (2015) High-Throughput Sequencing—The Key to Rapid Biodiversity Assessment of Marine Metazoa?. PLOS ONE 10(10): e0140342. https://doi.org/10.1371/journal.pone.0140342
Miljutina M.A., Miljutin D.M. (2015) A revision of the genus Paracanthonchus (Cyatholaimidae, Nematoda) with a tabular key to species and a description of P. mamubiae sp. n. from the deep North-Western Pacific. Deep-sea Research II 111, 104-118. https://doi.org/10.1016/j.dsr2.2014.08.002.
Meißner K., Götting M. (2015) Spionidae (Annelida: ‘Polychaeta’: Canalipalpata) from Lizard Island, Great Barrier Reef, Australia: the genera Malacoceros, Scolelepis, Spio, Microspio, and Spiophanes. Zootaxa, 4019: 378–413. https://doi.org/10.11646/zootaxa.4019.1.15
Knebelsberger T., Dunz A.R., Neumann D., Geiger M.F. (2015) Molecular diversity of Germany’s freshwater fishes and lampreys assessed by DNA barcoding. Molecular Ecology Resources, 15: 562–572. https://doi.org/10.1111/1755-0998.12322
Janssen A., Kaiser S., Meißner K., Brenke N., Menot L., Martínez Arbízu P. (2015) Reverse taxonomy reveals long-range distribution of abyssal species on polymetallic nodule fields: A comparison of the polychaete and isopod faunas between the French and German Exploration License Areas in the Clarion-Clipperton Zone (CCZ, NE-equatorial Pacific). PLOS ONE, 10(2): e0117790. https://doi.org/10.1371/journal.pone.0117790
Hofmann T., Raupach M.J., Martinez Arbízu P., Knebelsberger T. (2015) An application of in situ hybridization for the identification of commercially important fish species. Fisheries Research, 170: 1–8. https://doi.org/10.1016/j.fishres.2015.05.002
Gebhardt K., Knebelsberger T. (2015) Identification of cephalopod species from the North and Baltic Seas using morphology, COI and 18S rDNA sequences. Helgoland Marine Research, 69: 259–271. https://doi.org/10.1007/s10152-015-0434-7
Boeters H.D., Callot-Girardi H., Knebelsberger T. (2015) News of Pseudamnicola (Corrosella) of Spain and France (Mollusca: Gastropoda: Truncatelloidea). Folia Malacologica, 23: 95–119. https://doi.org/10.12657/folmal.023.007
2014
Brix S., Leese F., Riehl T., Kihara T.C. (2014) A new genus and new species of Desmosomatidae Sars, 1897 (Isopoda) from the east South-Atlantic abyss described by means of integrative taxonomy. Marine Biodiversity. https://doi.org/10.1007/s12526-014-0218-3
Vogt P., Miljutina M., Raupach M.J. (2014) The use of DNA sequence data for the identification of benthic nematodes from the North Sea. Helgoland Marine Research, 68: 549–558. https://doi.org/10.1007/s10152-014-0411-6
Raupach M.J., Bininda-Emonds O.R.P., Knebelsberger T., Laakmann S., Pfänder J., Leese F. (2014) Phylogeographic analysis of Ligia oceanica (Crustacea: Isopoda) reveals two deeply divergent mitochondrial lineages. Biological Journal of the Linnean Society, 112: 16–30. https://doi.org/10.1111/bij.12254
Meißner K., Bick A., Guggolz T., Götting M. (2014) Spionidae (Polychaeta: Canalipalpata: Spionida) from seamounts in the NE Atlantic. Zootaxa, 3786: 201–245. https://doi.org/10.11646/zootaxa.3786.3.1
Markhaseva E.L., Laakmann S., Renz J. (2014) An interim synopsis of the Bradfordian families with a description of Thoxancalanus spinatus (Copepoda: Calanoida), a new diaxid genus and species from the deep Atlantic Ocean. Marine Biodiversity, 44: 63–88. https://doi.org/10.1007/s12526-013-0185-0
Markert A., Raupach M.J., Segelken-Voigt A., Wehrmann A. (2014) Molecular identification and morphological characteristics of Asian brush-clawed crabs from native Japanese and invasive German sites: Hemigrapsus penicillatus (De Haan, 1835) versus H. takanoi Asakura & Watanabe 2005 (Crustacea: Brachyura). Organisms Diversity & Evolution, 14: 369–382. https://doi.org/10.1007/s13127-014-0176-4
Laakmann S., Holst S. (2014) Emphasizing the diversity of North Sea hydromedusae by combined morphological and molecular methods. Journal of Plankton Research, 36: 64–76. https://doi.org/10.1093/plankt/fbt078
Knebelsberger T., Thiel R. (2014) Identification of gobies (Teleostei: Perciformes: Gobiidae) from the North and Baltic Seas combining morphological analysis and DNA barcoding. Zoological Journal of the Linnean Society, 172: 831–845. https://doi.org/10.1111/zoj.12189
Knebelsberger T., Landi M., Neumann H., Kloppmann M., Sell A., Campbell P., Laakmann S., Raupach M.J., Carvalho G., Costa F. (2014) A reliable DNA barcode reference library for the identification of the European shelf fish fauna. Molecular Ecology Resources, 14: 1060–1071. https://doi.org/10.1111/1755-0998.12238
Khodami S., Martínez-Arbizu P., Stöhr S., Laakmann S. (2014) Molecular species delimitation of Icelandic brittle stars (Ophiuroidea). Polish Polar Research, 35: 243–260. https://doi: 10.2478/popore−2014−0011
Holst S., Laakmann S. (2014) Morphological and molecular discrimination of two closely related jellyfish species, Cyanea capillata and C. lamarckii (Cnidaria, Scyphozoa), from the northeast Atlantic. Journal of Plankton Research, 36: 48–63. https://doi.org/10.1093/plankt/fbt093
2013
Stöger I., Sigwart J.D., Kano Y., Knebelsberger T., Marshall B.A., Schwabe E., Schrödl M. (2013) The continuing debate on deep molluscan phylogeny: Evidence for Serialia (Mollusca, Monoplacophora + Polyplacophora). BioMed Research International, Article ID 407072. https://doi.org/10.1155/2013/407072
Lejzerowicz F., Esling P., Majewski W., Szczuciński W., Decelle J., Obadia C., Martínez Arbizu P.M., Pawlowski J. (2013) Ancient DNA complements microfossil record in deep-sea subsurface sediments. Biology Letters, 9(4). https://doi.org/10.1098/rsbl.2013.0283
Laakmann S., Gerdts G., Erler R., Knebelsberger T., Martínez Arbizu P., Raupach M.J. (2013) Comparison of molecular species identification for North Sea calanoid copepods (Crustacea) using proteome fingerprints and DNA sequences. Molecular Ecology Resources, 13: 862–876. https://doi.org/10.1111/1755-0998.12139
2012
Tang C.Q., Leasi F., Obertegger U., Kieneke A., Barraclough T.G., Fontaneto D. (2012) The widely used small subunit 18S rDNA molecule greatly underestimates true diversity in biodiversity surveys of the meiofauna. Proceedings of the National Academy of Sciences, 109: 16208–16212. https://doi.org/10.1073/pnas.1209160109
Kieneke A., Martínez Arbizu P.M., Fontaneto D. (2012) Spatially structured populations with a low level of cryptic diversity in European marine Gastrotricha. Molecular Ecology, 21: 1239–1254. https://doi.org/10.1111/j.1365-294X.2011.05421.x
Boeters H.D., Knebelsberger T. (2012) Revision of selected species of Bythinella Moquin-Tandon 1856 from Central Europe using morphology, anatomy and DNA barcodes (Caenogastropoda: Rissooidea). Archiv für Molluskenkunde, 141: 115–136. https://doi.org/10.1127/arch.moll/1869-0963/141/115-136
2011
Meißner K., Bick A., Bastrop R. (2011) On the identity of Spio filicornis (O.F. Müller, 1776) – with the designation of a neotype, and the description of two new species from the North East Atlantic Ocean based on morphological and genetic studies. Zootaxa, 2815: 1–27. https://doi.org/10.11646/zootaxa.2815.1.1
Gollner S., Fontaneto D., Martínez Arbizu P.M. (2011) Molecular taxonomy confirms morphological classification of deep-sea hydrothermal vent copepods (Dirivultidae) and suggests broad physiological tolerance of species and frequent dispersal along ridges. Marine Biology, 158: 221–231. https://doi.org/10.1007/s00227-010-1553-y
Brix S., Riehl T., Leese F. (2011) First genetic data for species of the genus Haploniscus Richardson, 1908 (Isopoda: Asellota: Haploniscidae) from neighbouring deep-sea basins in the South Atlantic. Zootaxa, 2838: 79–84.
2010
Knebelsberger T., Ditzler S., Laakmann S., Mohrbeck I., Raupach M.J. (2010) Molecular techniques for identifying North Sea fauna. In: Nimis P.L., Vignes Lebbe R. (eds.) Tools for identifying Biodiversity: progress and problems. Edizioni Università di Trieste, Trieste: 349.
Grabbert S., Renz J., Hirche H., Bucklin A. (2010) Species-specific PCR discrimination of species of the calanoid copepod Pseudocalanus, P. acuspes and P. elongatus, in the Baltic and North Seas. Hydrobiologia, 652: 289–297. https://doi.org/10.1007/s10750-010-0351-9
2009
Meißner K., Blank M. (2009) Spiophanes norrisi sp. nov. (Polychaeta: Spionidae) – a new species from the NE Pacific coast, separated from the Spiophanes bombyx complex based on both morphological and genetic studies. Zootaxa, 2278: 1–25. https://doi.org/10.11646/zootaxa.2278.1.1
Das MALDI-Labor am DZMB entwickelt und nutzt proteomisches Fingerprinting als innovative Infrastruktur für die Biodiversitätsforschung. Ziel ist es, Arten schnell, zuverlässig und reproduzierbar identifizieren zu können und damit eine zentrale Grundlage für ökologische, taxonomische und evolutionsbiologische Studien bereitzustellen. Im Mittelpunkt steht die Anwendung der MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization – Time of Flight) Massenspektrometrie, mit der charakteristische Proteinmuster von Organismen gemessen und für die Artbestimmung genutzt werden können.
Die Methode ermöglicht es, große Probenzahlen effizient zu analysieren und dadurch biologische Gemeinschaften mit hoher taxonomischer Auflösung zu erfassen. Insbesondere für individuenreiche und taxonomisch anspruchsvolle Organismengruppen eröffnet das proteomische Fingerprinting neue Möglichkeiten der Biodiversitätserfassung. Untersuchungen an marinen Plankton-, Benthos- und Meiofauna-Organismen zeigen, dass sowohl morphologisch gut unterscheidbare als auch kryptische Arten zuverlässig identifiziert werden können. Darüber hinaus lassen sich verschiedene Entwicklungsstadien vom Jungtier bis zum erwachsenen Tier, Populationsstrukturen und ökologische Muster in Lebensgemeinschaften quantitativ erfassen.
Ein zentraler Bestandteil der Arbeiten im MALDI-Labor ist die Entwicklung standardisierter Arbeitsabläufe für Probenahme, Fixierung, Lagerung und Messung. Diese methodischen Grundlagen sind entscheidend, um reproduzierbare Massenspektren zu erzeugen und langfristig nutzbare Referenzdatenbanken aufzubauen. Parallel dazu werden bioinformatische und statistische Verfahren entwickelt, die eine automatisierte Auswertung großer Spektrendatensätze ermöglichen. Machine-Learning-Ansätze tragen dazu bei, Identifikationsprozesse zu beschleunigen, Fehlzuordnungen zu minimieren und Biodiversitätsmuster auch ohne vollständige Referenzdatenbanken analysieren zu können.
Die Forschung im MALDI-Labor ist eng mit integrativer Taxonomie und ökologischen Anwendungen verknüpft. Proteomische Daten werden systematisch mit morphologischen und genetischen Informationen kombiniert, um Arten abzugrenzen, evolutionäre Beziehungen zu untersuchen und bislang verborgene Diversität sichtbar zu machen. Gleichzeitig entstehen offene methodische und analytische Werkzeuge, die eine breite Anwendung der Technologie in unterschiedlichen Organismengruppen und Ökosystemen ermöglichen. Damit trägt das Labor dazu bei, proteomisches Fingerprinting als leistungsfähige und skalierbare Methode in der Biodiversitätsforschung zu etablieren.
2026
Kelch, A., Spies, L., Franco, D.D., Nilsson, M.A., Rossel, S., Arbizu, P.M., Rauh, A., Stuckas, H., Kaiser, S., Brandt, A., 2026. Integrative taxonomy of mesosignidae Schultz, 1969 (Crustacea, Malacostraca) from North Pacific trenches: new species description and biogeographic insights from morphology, mtDNA, and proteomics. Progress in Oceanography 103682. https://doi.org/10.1016/j.pocean.2026.103682
2025
Neuhaus, J., de Wilt, M.E., Rossel, S., Brix, S., Etter, R.J., Jennings, R.M., Linse, K., Martínez Arbizu, P., Schwentner, M., Peters, J., 2025. High Connectivity at Abyssal Depths: Genomic and Proteomic Insights Into Population Structure of the Pan-Atlantic Deep-Sea Bivalve Ledella ultima (E. A. Smith, 1885). Ecology and Evolution 15, e71903. https://doi.org/10.1002/ece3.71903
2024
Stratmann, T., Busch, K., de Kluijver, A., Kelly, M., Mills, S., Rossel, S., Schupp, P.J., 2024. Nutrient fluxes, oxygen consumption and fatty acid composition from deep-water demo- and hexactinellid sponges from New Zealand. Deep Sea Research Part I: Oceanographic Research Papers 214, 104416. https://doi.org/10.1016/j.dsr.2024.104416
Rossel, S., Peters, J., Charzinski, N., Eichsteller, A., Laakmann, S., Neumann, H., Martínez Arbizu, P., 2024. A universal tool for marine metazoan species identification: towards best practices in proteomic fingerprinting. Sci Rep 14, 1280. https://doi.org/10.1038/s41598-024-51235-z
2023
Rossel, S., Peters, J., Laakmann, S., Martínez Arbizu, P., Holst, S., 2023. Potential of MALDI-TOF MS-based proteomic fingerprinting for species identification of Cnidaria across classes, species, regions and developmental stages. Molecular Ecology Resources 23, 1620–1631. https://doi.org/10.1111/1755-0998.13832
Rossel, S., Kaiser, P., Bode-Dalby, M., Renz, J., Laakmann, S., Auel, H., Hagen, W., Arbizu, P.M., Peters, J., 2023a. Proteomic fingerprinting enables quantitative biodiversity assessments of species and ontogenetic stages in Calanus congeners (Copepoda, Crustacea) from the Arctic Ocean. Molecular Ecology Resources 23, 382–395. https://doi.org/10.1111/1755-0998.13714
Peters, J., Laakmann, S., Rossel, S., Martínez Arbizu, P., Renz, J., 2023. Perspectives of species identification by MALDI-TOF MS in monitoring-Stability of proteomic fingerprints in marine epipelagic copepods. Mol Ecol Resour. https://doi.org/10.1111/1755-0998.13779
2022
Paulus, E., Brix, S., Siebert, A., Martínez Arbizu, P., Rossel, S., Peters, J., Svavarsson, J., Schwentner, M., 2022. Recent speciation and hybridization in Icelandic deep-sea isopods: An integrative approach using genomics and proteomics. Mol Ecol 31, 313–330. https://doi.org/10.1111/mec.16234
Korfhage, S.A., Rossel, S., Brix, S., McFadden, C.S., Ólafsdóttir, S.H., Martínez Arbizu, P., 2022. Species Delimitation of Hexacorallia and Octocorallia Around Iceland Using Nuclear and Mitochondrial DNA and Proteome Fingerprinting. Frontiers in Marine Science 9.
Kürzel, K., Kaiser, S., Lörz, A.-N., Rossel, S., Paulus, E., Peters, J., Schwentner, M., Martínez Arbizu, P., Coleman, C.O., Svavarsson, J., Brix, S., 2022. Correct Species Identification and Its Implications for Conservation Using Haploniscidae (Crustacea, Isopoda) in Icelandic Waters as a Proxy. Frontiers in Marine Science 8. https://doi.org/doi: 10.3389/fmars.2021.795196
Rossel, S., Uhlenkott, K., Peters, J., Vink, A., Martínez Arbizu, P., 2022. Evaluating species richness using proteomic fingerprinting and DNA barcoding—a case study on meiobenthic copepods from the Clarion Clipperton Fracture Zone. Mar. Biodivers. 52, 67. https://doi.org/10.1007/s12526-022-01307-y
2021
Renz, J., Markhaseva, E.L., Laakmann, S., Rossel, S., Martínez Arbizu, P., Peters, J., 2021. Proteomic fingerprinting facilitates biodiversity assessments in understudied ecosystems: A case study on integrated taxonomy of deep sea copepods. Molecular Ecology Resources.
2020
Rossel, S., Martínez Arbizu, P., 2020. Unsupervised biodiversity estimation using proteomic fingerprints from MALDI-TOF MS data. Limnology and Oceanography: Methods. https://doi.org/10.1002/lom3.10358
Wilke, T., Renz, J., Hauffe, T., Delicado, D., Peters, J., 2020. Proteomic Fingerprinting Discriminates Cryptic Gastropod Species. Malacologia 63, 131–137.
Rossel, S., Barco, A., Kloppmann, M., Martínez Arbizu, P., Huwer, B., Knebelsberger, T., 2020. Rapid species level identification of fish eggs by proteome fingerprinting using MALDI-TOF MS. Journal of Proteomics 103993.
2019
Rossel, S., Martínez Arbizu, P., 2019. Revealing higher than expected diversity of Harpacticoida (Crustacea: Copepoda) in the North Sea using MALDI-TOF MS and molecular barcoding. Scientific Reports 9, 9182. https://doi.org/10.1038/s41598-019-45718-7
Rossel, S., Khodami, S., Martínez Arbizu, P., 2019. Comparison of rapid biodiversity assessment of meiobenthos using MALDI-TOF MS and Metabarcoding. Frontiers in Marine Science 6, 659. https://doi.org/10.3389/fmars.2019.00659
Holst, S., Heins, A., Laakmann, S., 2019. Morphological and molecular diagnostic species characters of Staurozoa (Cnidaria) collected on the coast of Helgoland (German Bight, North Sea). Marine Biodiversity. https://doi.org/10.1007/s12526-019-00943-1
2018
Kaiser, P., Bode, M., Cornils, A., Hagen, W., Martínez Arbizu, P., Auel, H., Laakmann, S., 2018. High-resolution community analysis of deep-sea copepods using MALDI-TOF protein fingerprinting. Deep-Sea Research Part I: Oceanographic Research Papers 138, 122–130.
Rossel, S., Martínez Arbizu, P., 2018a. Automatic specimen identification of Harpacticoids (Crustacea:Copepoda) using Random Forest and MALDI-TOF mass spectra, including a post hoc test for false positive discovery. Methods in Ecology and Evolution 9, 1421–1434. https://doi.org/10.1111/2041-210X.13000
Rossel, S., Martínez Arbizu, P., 2018b. Effects of Sample Fixation on Specimen Identification in Biodiversity Assemblies based on Proteomic Data (MALDI-TOF). Frontiers in Marine Science 5, 149. https://doi.org/10.3389/fmars.2018.00149
2017
Bode, M., Laakmann, S., Kaiser, P., Hagen, W., Auel, H., Cornils, A., 2017. Unravelling diversity of deep-sea copepods using integrated morphological and molecular techniques. Journal of Plankton Research 39, 600–617.
2013
Laakmann, S., Gerdts, G., Erler, R., Knebelsberger, T., Martínez Arbizu, P., Raupach, M.J., 2013. Comparison of molecular species identification for North Sea calanoid copepods (Crustacea) using proteome fingerprints and DNA sequences. Molecular Ecology Resources 13, 862–76. https://doi.org/10.1111/1755-0998.12139
Die Dokumentation, Quantifizierung und Beschreibung morphologischer Merkmale von Arten bildet die unverzichtbare Grundlage für jede taxonomische, systematische und morphologische Forschung. Eine Vielzahl von bildgebenden Verfahren steht heute für die Untersuchung von marinen Protisten, Pflanzen und Tieren zur Verfügung.
Die konfokale Laserscanningmikroskopie (CLSM) ist ein ideales Werkzeug für die Untersuchung insbesondere von mikroskopischen Organismen aus marinen benthonischen und pelagischen Lebensräumen.
Das CLSM-Labor des Deutschen Zentrums für Marine Biodiversitätsfroschung (DZMB) wird derzeit häufig für die Untersuchung der äußeren Morphologie von Kleinkrebsen, sowie für die Rekonstruktion innerer Organsysteme von weichhäutigen Meiofauna-Taxa verwendet. Für diesen Zweck werden verschiedene histochemische und immun-histochemische Markierungsverfahren genutzt. Auch werden weitere Anwendungsbereiche und Präparationstechniken erschlossen und weiterentwickelt, wie beispielsweise die Verstärkung der kutikulären Autofluoreszens oder die Bildgebung und Untersuchung von mikro-bioerodierenden Organismen.
Das CLSM-Labor unserer Abteilung ist mit einem Leica® TCS SP5 System an einer DM5000B Mikroskopbasis ausgestattet. Sechs sichtbare Laser-Wellenlängen (458, 476, 488, 514, 561 und 633 nm), zwei Fluoreszenz-Detektoren und ein weiterer Detektor für den Durchlicht-Kanal stehen für unsere Experimente zur Verfügung. Die fluoreszierenden Objekte werden durch einen beugungsbegrenzt fokussierten Laserstrahl angeregt, der zeilenweise über die Probe gerastert wird. Die konfokale Anordnung zweier verstellbarer Blenden ermöglicht das Unterdrücken von Signalen, die nicht aus der Fokusebene stammen. Die schrittweise Änderung der Objekttischposition erlaubt schließlich die Erzeugung von dreidimensionalen Bildstapeln. Solche Datensätze können vielfältig verarbeitet werden, von der Erstellung tiefenscharfer Projektionsbilder bis hin zu dreidimensionalen Rekonstruktionen der äußeren Morphologie und inneren Anatomie der untersuchten Organismen, Quantifizierungen wie die Vermessung des Biovolumens von Organsystemen eingeschlossen.
93. Paulus, E., Brix, S., Siebert, A., Martínez Arbizu, P., Sven Rossel, S., Peters, J., Svavarsson, J., Schwentner, M. (2022): Recent speciation and hybridization in Icelandic deep-sea isopods: An integrative approach using genomics and proteomics. Molecular Ecology 31: 313-330. https://doi.org/10.1111/mec.16234
92. Kunze, M., Khodami, S., Ostmann, A., Packmor, J., George, K.H. (2026) Redescription of Enhydrosoma sarsi (Scott, 1905) (Copepoda, Harpacticoida, Cletodidae T. Scott) from the western Baltic Sea (Germany) and remarks on the systematics of Enhydrosoma Boeck, 1873. Zootaxa 5768: 335-369. https://doi.org/10.11646/zootaxa.5768.3.2
91. Minowa, A.K., Araújo, T.Q., Garraffoni, A.R.S., Kieneke, A. (2025): A threefold, specimen-saving morphological protocol for aquatic micrometazoans: proof of concept with an integrative re-investigation of the rare freshwater gastrotrich Dichaetura surreyi Martin, 1990. Zoomorphology 144: 61. https://doi.org/10.1007/s00435-025-00748-w
90. Kuru, S., Martínez Arbizu, P.M., Rossel, S. (2025): Revealing high genetic divergence masked by low morphological variability in harpacticoid genus Leptastacus Scott T., 1906 (Copepoda, Harpacticoida, Leptastacidae) including the description of five new species. Marine Biodiversity 55: 113. https://doi.org/10.1007/s12526-025-01583-4
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82. Biniek, K., Hendrycks, E, Jażdżewska, A.M. (2026). A new species of Pardalisca Krøyer, 1842 (Crustacea, Amphipoda, Pardaliscidae) from the Clarion-Clipperton Zone in the abyssal central east Pacific. ZooKeys 1274: 215-228. DOI: 10.3897/zookeys.1274.140692
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80. Wróblewski, B., Jażdżewska, A.M. (2026). A new species of Lepidepecreum Spence Bate & Westwood, 1868 (Crustacea, Amphipoda, Tryphosidae) from the Clarion-Clipperton Zone in the abyssal east Pacific. ZooKeys 1274: 141-159. DOI: 10.3897/zookeys.1274.127368
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78. Tandberg, A.H.S, Hughes, L.E. (2026). Stilipedidae Holmes, 1908 (Crustacea, Amphipoda) of the Clarion Clipperton Zone. ZooKeys 1274: 17-34. DOI: 10.3897/zookeys.1274.141416
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