Proteomic Laboratory

Proteome fingerprinting using MALDI-TOF MS

MALDI-TOF MS means Matrix Assissted Laser Desorption/Ionization Time Of Flight Mass Spectrometry. This method uses a matrix in which analytes are embedded, which then is evaporated using a laser. Hence, analytes are set free and accelerated by an electric field through a time-of-flight tube towards a detector. Due to different time-of-flight, analytes are separated by size. Using a matrix to embed analytes, this method allows measurement of macromolecules such as peptides and proteins without destroying them due to laser radiation. The measured peptides and proteins then result in the so called proteome fingerprint. In microbiology this method is already an established identification method to differentiate bacteria, viruses or fungi. Recently, this method was also applied successfully for identification of metazoan species.

In out section we are working to prepare this method for use in biodiversity assessments where specimen identification takes most of the time within a study. That is why we are testing this method in pilot studies for a variety of animal species. Additionally to that, we are working on methods allowing rapid analyses of MALDI-TOF MS data. Using MLADI-TOF MS we aim at reducing the time necessary for species identification in biodiversity assessments. In contrast to methods such as DNA barcoding only very few preparation steps are needed from a sorted specimen to the mass spectrum used for species identification. Aside from the time saved using this method in contrast to DNA barcoding, also the costs are a lot lower, making it interesting for animal groups where lots of specimens have to be identified to species level such as meiofauna research or analyses of plankton time series. The method also allows identification of cryptic species, making it an interesting tool also for makro- or megafauna species aiding in identification of hard to discern species.

Microarthridion littorale (Poppe, 1881) is a harpacticoid copepod frequently found in the North Sea. The blue line depicts a mass spectrum as it is generated using MALDI-TOF MS. Especially for such small species (500-700 µm) identification using MALDI-TOF MS would be interesting and helpful.