DZMB Bilder

Metabarcoding and NGS Laboratory


Die Charakterisierung der Meiofauna und der planktonischen Gemeinschaften der marinen Ökosysteme war aufgrund der Kleinheit der Individuen, der hohen Diversität der Gruppen und des Mangels an taxonomischen Spezialisten immer ein schwieriger und zeitaufwändiger Prozess. Die Anwendung von DNA-Barcoding in Verbindung mit modernen Sequenzierungstechniken (Next Generation Sequencing) stellt einen großen Fortschritt zur schnellen Einschätzung der Biodiversität dieser Organismen dar.

Diese Organismengruppen werden für die Überwachung der Gesundheit der Ökosysteme und die Bewertung der Auswirkungen menschlicher Aktivitäten immer wichtiger.

Unser Ziel ist es, schnelle Methoden zur Bewertung der biologischen Vielfalt zu entwickeln und zu testen. Diese sollen zum Verständnis der Veränderungen von Ökosystemen beitragen und gleichzeitig die Bearbeitungszeit und -kosten reduzieren. In unserem NGS-Lab, das mit einem Illumina MiSeq Sequenzierer ausgestattet ist, wenden wir Methoden des DNA-Metabarcodings und der eDNA für ganze Organismengemeinschaften an. Auf dem Gebiet des Metabarcodings arbeiten wir an der Optimierung der Verfahren zur DNA-Extraktion, der PCR und der Entwicklung von Bioinformatik-Pipelines zum Trimmen, Filtern und Analysieren der Illumina Reads und zur Zuordnung taxonomischer Identifikationen zu den mutmaßlichen OTUs (operational taxonomic units) oder Sequenzvarianten. In diesem Zusammenhang konzentriert sich unsere Forschung hauptsächlich auf die beiden häufig verwendeten Genfragmente mtDNA COI-Barcode und die hypervariable Region V1 & V2 der 18S rRNA, um die Diversität abzuschätzen und die Genauigkeit der Pipeline sowie die Fähigkeit dieser Marker zur Bewertung der Fauna zu untersuchen. Um eine bessere Identifizierung der Sequenzen zu erhalten, stellen wir unsere eigenen Referenzbibliotheken her, indem wir taxonomisch identifizierte Exemplare mit Hilfe des Sanger-Sequenzing mit einem Barcode versehen. Die genaue taxonomische Arbeit ist bei diesem Schritt, für den wir eng mit Spezialisten zusammenarbeiten, von entscheidender Bedeutung. Wir nutzen diese Bibliotheken in Kombination mit öffentlich zugänglichen NCBI-Repositorien.

Das Metabarcoding-Labor führt auch Analysen von Mikrobiomen durch, die mit Zielorganismen der Makro- und Megafauna wie Tiefseeschwämmen und Crustaceen assoziiert sind.

Zusätzlich verwenden wir restriktionsassoziierte DNA-Marker (in unserem Labor verwenden wir 2bRADs), um die genetische Konnektivität zwischen Populationen mariner Arten für verschiedene Projekte zu untersuchen.

Leitung

Team Metabarcoding Khodami
Dr. Sahar Khodami
Leitung Metabarcoding and NGS Laboratory

Link zum Fachbereich Metabarcoding und NGS Labor

Link Metabarcoding and NGS Laboratory

EDUCATIONS
• Carl von Ossietzky Universität Oldenburg, Institute of Biology, Oldenburg, Germany.
Ph.D. in Marine Biology
2018
Dissertation Title: Molecular Phylogeny and Revision of Copepod Orders (Crustacea:
Copepoda)

• University of Tehran, Science and Research Branch, Department of Fishery, Tehran, Iran.
M.Sc. Marine Biology, Fishery
2009

• University of Mazandaran, Badeleh Branch, Department of Aquaculture and Fisheries Engineering, Sari, Iran.
B.Sc. Biology
2006

FURTHER EDUCATIONS
• In the framework of the Expert-in-training programme 2012-2013 of the Distributed European School of Taxonomy (DEST) followed the training course “Phylogenetic and Systematic and Molecular Dating” at the University of Copenhagen (Denmark) from 21 January -1 February 2013. Winner of the DEST-grant.
• Workshop on statistic methods in phylogeny with „R“ provided by Gesellschaft für Biologische Systematik (GfBS)” 24th -25th February 2016. Munich. Germany.
• MiSeq training provided by Illumina Inc. 22 – 23rd June 2017. In Senckenberg Am Meer, DZMB department, Wilhelmshaven, Germany.

PUBLICATIONS
• Khodami S., Mercado-Salas N.F., Tang D., Martinez Arbizu P. 2019. Molecular evidence for the retention of the Thaumatopsyllidae in the order Cyclopoida (Copepoda) and establishment of four suborders and two families within the Cyclopoida. Molecular Phylogenetics and Evolution, 138: 43-52.
• Khodami S., McArthur V., Blanco-Bercial L. and P. Martinez Arbizu. 2017. Molecular phylogeny and revision of copepod orders (Crustacea: Copepoda). Scientific Reports, 7: 9164 (DOI: 10.1038/s41598-017-06656-4).
• Khodami S., Martinez Arbizu P., Stöhr S. and S. Laakmann. 2014, Molecular species delimitation of Icelandic brittle stars (Ophiuroidea). Polish Polar Research, 35 (2): 243-260.
• Mercado Salas N. F., Khodami S., Martinez Arbizu P. and M. Elías-Gutiérrez. 2019. Integrative taxonomy of the genus Mastigodiaptomus, description of a new species from Sian kaan Biosphere Reserve in the Yucatan Peninsula and comments of the genetic structure and distributional patterns of the genus in Mexico. Arthropod Systematics and Phylogeny, 76(3):487-507
• Mercado-Salas N. F., Khodami S. & Martínez Arbizu P. 2019. Convergent evolution of mouthparts morphology between Siphonostomatoida and a new genus of deep-sea Aegisthidae Giesbrecht, 1893 (Copepoda: Harpacticoida). Marine Biodiversity: 1-21. https://doi.org/10.1007/s12526-018-0932-3
• Gollner S., Stuckas H., Kihara T.C., Laurent S., Khodami S. and P. Martinez Arbizu. 2016. Mitochondrial DNA analyses indicate high diversity, expansive population growth and high genetic connectivity of vent copepods (Dirivultidae) across different oceans. PLoS ONE, 11 (10): e0163776. doi:10.1371/journal.
• Glover A., Dahlgren T., Taboada S., Paterson G., Wiklund H., Waeschenbach A., Cobley A., Martínez P., Kaiser S., Schnurr S., Khodami S., Raschka U., Kersken D., Stuckas H., Menot L., Bonifacio P., Vanreusel A., Macheriotou L., Cunha M., Hilário A., Rodrigues C., Colaço A., Ribeiro P., Błażewicz M., Gooday A., Jones D., Billett D., Goineau A., Amon D., Smith C., Patel T., McQuaid K., Spickermann R. and S. Brager. 2016. The London Workshop on the Biogeography and Connectivity of the Clarion-Clipperton Zone. Research Ideas and Outcomes 2: e10528. doi: 10.3897/rio.2.e10528

REPORTS:
• Ostmann, A., Khodami S., Schnurr S., Bauernfeld W., Yasuhara M. and P. Martinez. 2012, Meiofauna communities around Icelandic waters. In S. Brix et al.: Overflow, Circulation, and Biodiversity Cruise No. 85, Leg 3 August 27 – September 28, 2011 Reykjavik (Iceland) – Cuxhaven (Germany). Meteor – Berichte.
• Vanreusel A., Macheriotou L., Khodami S., Raschka U., and P. Martinez Arbizu. 2015, Metazoan meiofauna. In P. Martinez Arbizu et al. : SO239 EcoResponse, Assessing the Ecology, Connectivity and Resilience of Polymetallice Nodule field Systems. Balboa (Panama)- Manzanillo (Mexico), 11th March – 30th April 2015. SONNE – Fahrtberichte.

CONFERENCES
Oral
• Khodami S., Mercado-Salas N.F. and P. Martinez Arbizu. Gradual morphological adaptation to deep sea and pelagic habitats in Aegisthidae Giesbrecht, 1893 (Copepoda: Harpacticoida) inferred from genus level molecular phylogeny. 17th Meiofauna Conference. July 7-13, 2019. Évora. Portugal.
• Khodami S., Mercado-Salas N.F. and P. Martinez Arbizu. 2019. New insights to the phylogeny of Misophrioida (Copepoda), inferred from nuclear and mitochondrial DNA. 4 ISAE: 4th International Symposium for Anchialine Ecosystems. 1-7 October, 2018. Lanzarote Canarias.
• Khodami S., McArthur V., Blanco-Bercial L. and P. Martinez Arbizu. Molecular phylogeny and revision of Copepod. 13ICOC: 13 International Copepoda Conference 2017. Los Angeles. The U.S.A.
• Khodami S., Mercado-Salas N.F., and P. Martinez Arbizu. Molecular phylogeny of Aegisthidae Giesbrecht, 1893 (Copepoda: Harpacticoida) reveals quick morphological adaptation to deep-sea and pelagic habitats. 13ICOC (13 International Copepoda Conference 2017). Los Angeles. The U.S.A.
• Khodami S. and P Martinez Arbizu. First parasitic Aegisthidae Giesbrecht, 1893 (Copepoda: Harpacticoida) showing convergent evolution with Siphonostomatoida and its phylogenetic position within the family. 16th International Meiofauna Conference (ISIMCO 2016). Crete. Greece.
• Mercado-Salas N.F., Khodami S., and P. Martinez Arbizu. How diverse are bromeliad copepods and ostracods in the tropical forest of the Yucatan Peninsula? 13ICOC: 13 International Copepoda Conference 2017. Los Angeles. The U.S.A.
• Khodami S. and P. Martinez Arbizu. A new phylogeny of copepod (Crustacean) orders based on 18S rDNA. 17th Annual Meeting of the Gesellschaft für Biologische Systematik (GfBS) “Taxa in Time and Space” 2016. Munich. Germany.
• Khodami S. and P. Martinez Arbizu. Phylogenetic relationship of Copepoda orders based on 18S rDNA. 12ICOC: 12 International Copepoda Conference 2014). Seoul. Korea.
• Mercado-Salas N.F., Khodami S., Martinez A. and P. Martinez Arbizu. Diversity and phylogeny of anchialine cave copepods. 17th Meiofauna Conference. July 7-13, 2019. Évora. Portugal.
• Martinez Arbizu P., Khodami S. and N.F. Mercado-Salas. The Senckenberg Metabarcoding Pipeline: Examples from Meiofauna and Plankton. Marine ‘omics Workshop. July 16-17, 2018. Delmenhorst, Germany.

Poster
• Khodami S., Mercado-Salas N.F. and Martinez Arbizu P. An updated phylogeny and systematics of Cyclopoida (Copepoda). 17th Meiofauna Conference. July 7-13, 2019. Évora. Portugal.
• Khodami S., Rossel S. and P. Martinez Arbizu Comparison of Metabarcoding and MALDI-TOF for rapid biodiversity assessment of Harpacticoid Copepods (Crustacea) on a tidal flat (Hooksiel, Germany). Marine ‘omics Workshop. July 16-17, 2018. Delmenhorst, Germany.
• Mercado-Salas N.F., Khodami S., and P. Martinez Arbizu. Reference Library and Metabarcoding of Red Sea Plankton. Marine ‘omics Workshop. July 16-17, 2018. Delmenhorst, Germany.

EXPEDITIONS
• SO262 Mangan 2018 Expedition (Guayaquil- Suva). Geology, Biodiversity and environmental of the German License Area for the Exploration of Polymetallic Nodules in the Equatorial NE Pacific. 4th April – 29th May 2018 on board of RV SONNE. (SO262).
• SO239 EcoResponse Expedition (Balboa – Manzanillo). Assessing the Ecology, Connectivity, and Resilience of Polymetallic Nodule Field Systems. 11th March – 30th April 2015 on board of RV SONNE. (SO239).
• IceAGE1 Expedition (Reykjavik – Cuxhaven). Icelandic Marine Animals: Genetics and Ecology. 19th August – 28th September 2011 on board of RV Meteor (ME 85/3).

Articles of Metabarcoding and NGS Laboratory

2020
69. JANINA RAHLFF, SAHAR KHODAMI, LISA VOSKUHL, MATTHEW P. HUMPHREYS, CHRISTIAN STOLLE, PEDRO MARTINEZ ARBIZU, MARIANA RIBAS-RIBAS.
(Submitted 2020). Short-term responses to ocean acidification: effects on relative abundance of eukaryotic plankton from the tropical Timor Sea.

68. GEORG STEINERT, KATHRIN BUSCH, KRISTINA BAYER, SAHAR KODAMI, PEDRO MARTINEZ ARBIZU, MICHELLE KELLY, SADIE MILLS, DIRK ERPENBECK, MARTIN DOHRMANN, GERT WÖRHEIDE, UTE HENTSCHEL AND PETER J. SCHUPP. (April 2020 accepted, in proof). Compositional and Quantitative Insights Into Bacterial and Archaeal Communities of South Pacific Deep-Sea Sponges (Demospongiae and Hexactinellida). Frontiers in microbiology. doi: 10.3389/fmicb.2020.00716

67. CHRISTODOULOU M., O’HARA T., HUGALL A., KHODAMI S., RODRIGUES C.F., HILARIO A., VINK A., MARTINEZ ARBIZU P. (2020). Unexpected high abyssal ophiuroid diversity in polymetallic nodule fields of the northeast Pacific Ocean and implications for conservation. Biogeosciences, 17, 1–32. https://doi.org/10.5194/bg-17-1-2020 

66.  NANCY F. MERCADO-SALAS, SAHAR KHODAMI AND P. MARTÍNEZ ARBIZU. (submitted) Hitchhiking between bromeliads: a study based on copepods and ostracods from the Yucatan Peninsula, Mexico.

2019
65. ROSSEL, S, KHODAMI S. MARTINEZ P.
(2019). Comparison of rapid biodiversity assessment of meiobenthos using MALDI-TOF MS and Metabarcoding”, DOI: 10.3389/fmars.2019.00659. Journal of Frontiers in Marine Science.

64. CHRISTODOULOU M., O’HARA T., HUGALL A.F., MARTINEZ ARBIZU P. (2019). Dark Ophiuroid Biodiversity in a Prospective Abyssal Mine Field. Current Biology 29, 1–4. https://doi.org/10.1016/j.cub.2019.09.012

63. ROSSEL, S. AND MARTÍNEZ ARBIZU, P. (2019) Revealing higher than expected diversity of Harpacticoida (Crustacea:Copepoda) in the North Sea using MALDI-TOF MS and molecular barcoding. Scientific Reports. DOI: 10.1038/s41598-019-45718-7

62. KHODAMI S., MERCADO-SALAS N.F., AND MARTÌNEZ ARBIZU P. (2020) Genus level molecular phylogeny of Aegisthidae Giesbrecht, 1893 (Copepoda: Harpacticoida) reveals morphological adaptation to deep sea and pelagic habitats. BMC Evolutionary Biology.

61. KHODAMI, S., MERCADO-SALAS, N.F., TANG, D., MARTINEZ ARBIZU,P. (2019) Molecular evidence for the retention of the Thaumatopsyllidae in the order Cyclopoida (Copepoda) and establishment of four suborders and two families within the Cyclopoida. Molecular Phylogenetics and Evolution, 138:43-52.

60. MERCADO-SALAS ET AL. (2018) Integrative taxonomy of the genus Mastigodiaptomus (Copepoda), description of a new species from the Yucatan Peninsula and comments on the genetic structure and distributional patterns of the genus in Mexico. Arthropod Systematics and Evolution

59. SABINE HOLST, ANNEKE HEINS, SILKE LAAKMANN.(2019). Morphological and molecular diagnostic species characters of Staurozoa (Cnidaria) collected on the coast of Helgoland (German Bight, North Sea). 10013/epic.4f95b184-bb12-44fe-943d-b0f989f1bf93

2018
58. CHRISTODOULOU M. & CRISTINA KIHARA T.
(2018). Lectotype designation and distribution updates on the freshwater shrimp species Atyaephyra stankoi Karaman 1972. Zootaxa 4531 (1): 123–133. https://doi.org/10.11646/zootaxa.4531.1.7.

57. RENZ, J. , MARKHASEVA, E. L. AND LAAKMANN, S. (2018): The phylogeny of Ryocalanoidea (Copepoda, Calanoida)based on morphology and a multi-gene analysis with a description of new ryocalanoidean species , Zoological Journal of the Linnean Society, pp. 1-33 . doi: 10.1093/zoolinnean/zly069

56. LAAKMANN, S., MARKHASEVA, E.L., RENZ, J. Do molecular phylogenies unravel the relationships among the evolutionary young “Bradfordian” families (Copepoda; Calanoida)? Molecular Phylogenetics and Evolution –submitted

55. ROSSEL, S. & MARTINEZ ARBIZU, P. (2018) Effects of Sample Fixation on Specimen Identification in Biodiversity Assemblies Based on Proteomic Data (MALDI-TOF). Frontiers in Marine Science 5:149. DOI: 10.3389/fmars.2018.00149

54. Convergent evolution of mouthparts morphology between Siphonostomatoida and a new genus of deep-sea Aegisthidae Giesbrecht, 1893 (Copepoda Harpacticoida). MERCADO SALAS, N. F., KHODAMI, S & P. MARTINEZ ARBIZU. Marine Biodiversity

53. „Metabarcoding of marine environmental DNA based on mitochondrial and nuclear genes“ BY BABETT GÜNTHER, THOMAS KNEBELSBERGER, HERMANN NEUMANN, SILKE LAAKMANN, AND PEDRO MARTINEZ ARBIZU [Paper #SREP-17-55965]

52. HOLST S, LAAKMANN S (submitted) First record of the stalked jellyfish Haliclystus tenuis Kishinouye, 1910 (Cnidaria: Staurozoa) in Atlantic waters. Marine Biodiversity

51. MERTENS, K.N., CARBONELL-MOORE, M.C., POSPELOVA, V., HEAD, M.J., HIGHFIELD, A., SCHROEDER, D., GU, H., ANDREE, K.B., FERNÁNDEZ, M., YAMAGUCHI, A., TAKANO, Y., MATSUOKA, K., NÉZAN, E., BILIEN, G., OKOLODKOV, Y., KOIKE, K., HOPPENRATH, M., PFAFF, M., PITCHER, G., AL-MUFTAH, A., ROCHON, A., LIM, P.T., LEAW, C.P., LIM, Z.F. & ELLEGAARD, M. (2018): The potentially toxic Pentaplacodinium saltonense gen. et sp. nov. (Dinophyceae), and its relationship to the cyst-defined Operculodinium psilatum and the yessotoxin-producing Protoceratium reticulatum. Harmful Algae 71: 57-77

2017
50.  BRIX, S., LEESE, F., RIEHL, T., KIHARA, T.C.
(2017). A new genus and new species of Desmosomatidae Sars, 1897 (Isopoda) from the east South-Atlantic abyss described by means of integrative taxonomy. Marine Biodiversity.

49. TILLMANN, U., HOPPENRATH, M., GOTTSCHLING, M., KUSBER, W.-H. & ELBRÄCHTER, M. (2017): Plate pattern clarification of the marine dinophyte Heterocapsa triquetra sensu Stein (Dinophyceae) collected at the Kiel Fjord (Germany). Journal of Phycology 53: 1305-1324.

48. LAAKMANN S, BOOS K,  KNEBELSBERGER T, RAUPACH MJ, NEUMANN H (2017) Species identification of echinoderms from the North Sea by combining morphology and molecular data. Helgoland Marine Research 70: 18.

47. KIENEKE, A. & NIKOUKAR, H. (2017): Integrative morphological and molecular investigation of Turbanella hyalina Schultze, 1853.(Gastrotricha: Macrodasyida), including a redescription of the species. Zoologischer Anzeiger 267: 168-186.

46. KHODAMI, S.; MCARTHUR, J. V.; BLANCO-BERCIAL, L. & MARTÍNEZ ARBIZU, P. M. (2017): Molecular Phylogeny and Revision of Copepod Orders (Crustacea: Copepoda), SCIENTIFIC REPORTS 7.

45. HOPPENRATH M. , YUBUKI N. , STERN R. &  LEANDER B.S. (2017): Ultrastructure and molecular phylogenetic position of a new marine sand-dwelling dinoflagellate from British Columbia, Canada: Pseudadenoides polypyrenoides sp. nov. (Dinophyceae). European Journal of Phycology 52(2): 208-224.

44. HOFMANN, T., KNEBELSBERGER, T. KLOPPMANN, M., ULLEWEIT, J., RAUPACH, M.J. (2017) Egg identification of three economical important fish species using DNA barcoding in comparison to a morphological determination. Journal of Applied Ichthyology 33(5): 925-932.

43. BODE, M., LAAKMANN, S., KAISER, P., HAGEN, W., AUEL, H., CORNILS, A. (2017) Unraveling diversity of deep-sea copepods using integrated morphological and molecular techniques. Journal of Plankton Research 1-18. DOI: 10.1093/plankt/fbx031.

2016
42. THIEL, R., KNEBELSBERGER, T.
(2016) How reliably can northeast Atlantic sand lances of the genera Ammodytes and Hyperoplus be distinguished? A comparative application of morphological and molecular methods. ZooKeys (617): 139.

41. SCHADE, F.M., RAUPACH, M.J., WEGNER, K.M. (2016) Seasonal variation in parasite infection patterns of marine fish species from the Northern Wadden Sea in relation of interannual temperature fluctuations. Journal of the Sea 113: 73-84.

40. RAUPACH, M.J., AMANN, R., WHEELER, Q.D., ROOS, C. (2016) The application of “omics”-technologies for the classification and identification of animals. Organisms, Diversity & Evolution 16: 1-12.

39. OLIVEIRA, L. M., KNEBELSBERGER, T., LANDI, M., SOARES, P., RAUPACH, M. J., & COSTA, F. O. (2016) Assembling and auditing a comprehensive DNA barcode reference library for European Marine fishes. Journal of Fish Biology 89(6) 2741-2754.

38. MILJUTIN D., MILJUTINA M. (2016) Intraspecific variability of morphological characters in the species-rich deep-sea genus Acantholaimus (Nematoda: Chromadoridae). Nematology 18, 455-473.

37. MEISSNER, K., BICK, A., GÖTTING, M. (2016). Arctic Pholoe (Polychaeta, Pholoidae): when integrative taxonomy helps to sort out barcodes. Zoological Journal of the Linnean Society.

36. LAAKMANN, S., BOOS, K., KNEBELSBERGER, T., RAUPACH, M.J., NEUMANN, H. (2016) Species identification of echinoderms from the North Sea by combining morphology and molecular data. Helgoland Marine Research 70:18. DOI: 10.1186/s10152-016-0468-5

35. GÜNTHER, B., RAUPACH, M.J., KNEBELSBERGER, T. (2016) Full-length and mini-length DNA barcoding for the identification of seafood commercially traded in Germany. Food Control  73, 922-929.

34. BARCO, A., RAUPACH, M.J., LAAKMANN, S., NEUMANN, H., KNEBELSBERGER, T. (2016) Identification of North Sea molluscs with DNA barcoding. Molecular Ecology Resources 16 (1): 288-297.

2015
33. RAUPACH, M.J., BARCO, A., STEINKE, D., BEERMANN, J., LAAKMANN, S., MOHRBECK, I., NEUMANN, H., KIHARA, T.C., POINTNER, K., RADULOVICI, A., SEGELKEN-VOIGT, A., WESSE, C., KNEBELSBERGER, T.
(2015) The application of DNA barcodes for the identification of marine crustaceans from the North Sea and adjacent regions. Public Library of Science ONE 10 (9): e0139421.

32. RAUPACH, M.J., BARCO, A., BEERMANN, J., KIENEKE, A., LAAKMANN, S., MOHRBECK, I., NEUMANN, H., KNEBELSBERGER, T. (2015) Crabs, scallops, fish, and more: barcoding the marine fauna of the North Sea. Genome 58, 270.

31. RAUPACH, M.J. & RADULOVICI, A.E. (2015) Looking back on a decade of barcoding crustaceans. ZooKeys 539: 53-81.30. MOHRBECK I, RAUPACH MJ, MARTÍNEZ ARBIZU P, KNEBELSBERGER T, LAAKMANN S (2015) High-throughput sequencing – The Key to Rapid Biodiversity Assessment of Marine Metazoa? Public Library of Science ONE 10(10): e0140342.

29. MILJUTINA M.A., MILJUTIN D.M. (2015) A revision of the genus Paracanthonchus (Cyatholaimidae, Nematoda) with a tabular key to species and a description of P. mamubiae sp. n. from the deep North-Western Pacific. Deep-sea Research II 111, 104-118.

28. MEIßNER, K. & GÖTTING, M. (2015). Spionidae (‘Polychaeta’: Canalipalpata) from Lizard Island (Great Barrier Reef, Australia): the genera Malacoceros, Scolelepis, Spio, Microspio, and Spiophanes. Zootaxa [Special Issue] 4019 (1), 378-413.

27. KNEBELSBERGER, T., DUNZ, A.Z., NEUMANN, D., GEIGER, M.F. (2015) Molecular diversity of Germany`s freshwater fishes and lampreys assessed by DNA barcoding. Molecular Ecology Resources 15: 562-572.

26. JANSSEN, A., KAISER, S., MEISSNER, K., BRENKE, N., MENOT, L., MARTÍNEZ ARBÍZU, P. (2015). Reverse taxonomy reveals long-range distribution of abyssal species on polymetallic nodule fields: A comparison of the polychaete and isopod faunas between the French and German Exploration License Areas in the Clarion-Clipperton Zone (CCZ, NE-equatorial Pacific). PLoS ONE 10(2), e0117790.

25. HOFMANN, T., RAUPACH, M.J., MARTINEZ ARBÍZU, P., KNEBELSBERGER T. (2015) An application of in situ hybridization for the identification of commercially important fish species. Fisheries Research 170 (10): 1-8.

24. GEBHARDT, K., KNEBELSBERGER, T. (2015) Identification of cephalopod species from the North and Baltic Seas using morphology, COI and 18S rDNA sequences. Helgoland Marine Research 69 (3): 259-271.

23.  BOETERS, H.D., CALLOT-GIRARDI, H., KNEBELSBERGER, T. (2015) News of Pseudamnicola (Corrosella) in its distinct West European distribution area (Mollusca: Gastropoda: Truncatelloidea). Folia Malacologica 23: 95–119.

2014
22. VOGT, P., MILJUTINA, M. & RAUPACH, M.J.
(2014): The use of DNA sequence data for the identification of benthic nematodes from the North Sea. Helgoland Marine Research 68: 549-558.

21. RAUPACH, M.J., BININDA-EMONDS, O.R.P., KNEBELSBERGER, T., LAAKMANN, S., PFÄNDER, J., LEESE, F. (2014) Phylogeographic analysis of Ligia oceanica (Crustacea: Isopoda) reveals two deeply divergent mitochondrial lineages. Biological Journal of the Linnean Society 112: 16-30.

20. MEISSNER, K., BICK, A., GUGGOLZ, T., GÖTTING, M. (2014). Spionidae (Polychaeta: Canalipalpata: Spionida) from seamounts in the NE Atlantic. Zootaxa, 3786(3), 201-245.

19. MARKHASEVA, E.L., LAAKMANN, S., RENZ, J. (2014) An interim synopsis of the Bradfordian families with a description of Thoxancalanus spinatus (Copepoda: Calanoida), a new diaxid genus and species from the deep Atlantic Ocean. Marine Biodiversity 44: 63-88.

18. MARKERT, A., RAUPACH, M.J., SEGELKEN-VOIGT, A., WEHRMANN, A. (2014) Molecular identification and morphological characteristics of Asian brush-clawed crabs from native Japanese and invasive German sites: Hemigrapsus penicillatus (De Haan, 1835) versus H. takanoi Asakura & Watanabe 2005 (Crustacea: Brachyura). Organisms, Diversity and Evolution 14: 369-382.

17. LAAKMANN, S., HOLST, S. (2014) Emphasizing the diversity of North Sea hydromedusae by combined morphological and molecular methods. Journal of Plankton Research 36(1): 64-76.

16. KNEBELSBERGER, T., THIEL, R. (2014) Identification of gobies (Teleostei: Perciformes: Gobiidae) from the North and Baltic Seas combining morphological analysis and DNA barcoding. Zoological Journal of the Linnean Society 172: 831-845.

15. KNEBELSBERGER, T., LANDI, M., NEUMANN, H., KLOPPMANN, M., SELL, A., CAMPBELL, P., LAAKMANN, S., RAUPACH, M.J., CARVALHO, G., COSTA, F. (2014) A reliable DNA barcode reference library for the identification of the European shelf fish fauna. Molecular Ecology Resources 14: 1060-1071.

14. KHODAMI, S., MARTÍNEZ ARBIZU, P., STÖHR, S., LAAKMANN S. (2014) Molecular species delimination of Icelandic brittle stars (Ophiuroidea). Polish Polar Research 35: 243-260.

13. HOLST, S., LAAKMANN, S. (2014) Morphological and molecular discrimination of two closely related jellyfish species, Cyanea capillata and C. lamarckii (Cnidaria, Scyphozoa), from the northeast Atlantic. Journal of Plankton Research 36(1): 48-63.

2013
12. STÖGER, I., SIGWART, J. D., KANO, Y., KNEBELSBERGER, T., MARSHALL, B. A., SCHWABE, E., SCHRÖDL, M.
(2013) The continuing debate on deep molluscan phylogeny: Evidence for Serialia (Mollusca, Monoplacophora + Polyplacophora). BioMed Research. Article ID 407072.

11. LEJZEROWICZ, F.; ESLING, P.; MAJEWSKI, W.; SZCZUCINSKI, W.; DECELLE, J.; OBADIA, C.; MARTÍNEZ ARBIZU, P. M. & PAWLOWSKI, J. (2013), ‚Ancient DNA complements microfossil record in deep-sea subsurface sediments‘, BIOLOGY LETTERS 9(4).

10. LAAKMANN S, GERDTS G, ERLER R, KNEBELSBERGER T, MARTÍNEZ-ARBIZU P, RAUPACH MJ (2013) Comparison of molecular species identification for North Sea calanoid copepods (Crustacea) using proteome fingerprints and DNA sequences. Molecular Ecology Resources 13: 862-876.

2012
9. TANG, C.Q., LEASI, F., OBERTEGGER, U., KIENEKE, A., BARRACLOUGH, T.G., FONTANETO, D.
(2012): The widely used small subunit 18S rDNA molecule greatly underestimates true diversity in biodiversity surveys of the meiofauna. PNAS 109: 16208-16212.

8. KIENEKE, A., MARTÍNEZ ARBIZU, P.M., FONTANETO, D. (2012): Spatially structured populations with a low level of cryptic diversity in European marine Gastrotricha. Molecular Ecology 21: 1239-1254.

7. BOETERS, D. B. & KNEBELSBERGER, T. (2012) Revision of selected species of Bythinella MOQUIN-TANDON 1856 from Central Europe using morphology, anatomy and DNA barcodes (Caenogastropoda: Rissooidea). Archiv für Molluskenkunde 141 (1): 115-136.

2011
6. MEIßNER, K., BICK, A. & BASTROP, R.
(2011). On the identity of Spio filicornis (O.F. Müller, 1776) – with the designation of a neotype, and the description of two new species from the North East Atlantic Ocean based on morphological and genetic studies. Zootaxa 2815, 1-27.

5. GOLLNER, S.; FONTANETO, D. & MARTÍNEZ ARBIZU, P. M. (2011), ‚Molecular taxonomy confirms morphological classification of deep-sea hydrothermal vent copepods (Dirivultidae) and suggests broad physiological tolerance of species and frequent dispersal along ridges‘, MARINE BIOLOGY 158(1), 221-231.

4. BRIX, S., RIEHL, T. & LEESE, F. (2011) First genetic data for species of the genus Haploniscus Richardson, 1908 (Isopoda: Asellota: Haploniscidae) from neighbouring deep-sea basins in the South Atlantic. Zootaxa. 2838: 79–84.

2010
3. KNEBELSBERGER, T., DITZLER, S., LAAKMANN, S., MOHRBECK, I., RAUPACH, M.J.
(2010) Molecular techniques for identifying North Sea fauna. In: Nimis, P.L., Vignes Lebbe, R. [Eds.]: Tools for identifying Biodiversity: progress and problems, Edizioni Universita di Trieste, Trieste, 349.

2. GRABBERT, S., RENZ, J., HIRCHE, H.-J., BUCKLIN, A. (2010) Species-specific PCR discrimination of species of the calanoid copepod Pseudocalanus, P. acuspes and P. elongatus, in the Baltic and North Seas. Hydrobiologia 652: 289-297

2009
1. MEIßNER, K. & BLANK, M.
(2009). Spiophanes norrisi sp. nov. (Polychaeta: Spionidae) – a new species from the NE Pacific coast, separated from the Spiophanes bombyx complex based on both morphological and genetic studies. Zootaxa 2278, 1-25.